Papers describing techniques used in Pichia Expression (chronological list)
- Vogl T, Hartner FS, Glieder A. New opportunities by synthetic biology for biopharmaceutical production in Pichia pastoris. Curr Opin Biotechnol. 2013 Mar 19 [Epub ahead of print] PMID: 23522654.
- Chen MT, Lin S, Shandil I, Andrews D, Stadheim TA, Choi BK. Generation of diploid Pichia pastoris strains by mating and their application for recombinant protein production. Microb Cell Fact. 2012 Jul 2;11(1):91. [Epub ahead of print] PMID: 22748191.
- Logez C, Alkhalfioui F, Byrne B, Wagner R. Preparation of Pichia pastoris expression plasmids. Methods Mol Biol. 2012;866:25-40. PMID: 22454111.
- Schaefer JV, Plückthun A. Engineering aggregation resistance in IgG by two independent mechanisms: lessons from comparison of Pichia pastoris and mammalian cell expression. J Mol Biol. 2012 Apr 6;417(4):309-35. Epub 2012 Jan 27. PubMed PMID: 22306407.
- Lin S, Houston-Cummings NR, Prinz B, Moore R, Bobrowicz B, Davidson RC, Wildt S, Stadheim TA, Zha D. A novel fragment of antigen binding (Fab) surface display platform using glycoengineered Pichia pastoris. J Immunol Methods. 2012 Jan 31;375(1-2):159-65. Epub 2011 Oct 12. PubMed PMID: 22019510.
- Du M, Battles MB, Nett JH. A color-based stable multi-copy integrant selection system for Pichia pastoris using the attenuated ADE1 and ADE2 genes as auxotrophic markers. Bioeng Bugs. 2012 Jan 1;3(1):32-7. doi: 10.4161/bbug.3.1.17936. Epub 2012 Jan 1. PubMed PMID: 22126802.
- Yang J, Yan X, Huang R, Zhang B. Codon optimization, expression and enzymatic comparison of Rhizopus oryzae lipases pro-ROL and m-ROL in Pichia pastoris. Sheng Wu Gong Cheng Xue Bao. 2011 Dec;27(12):1780-8. PMID: 22506419.
- Akcapinar GB, Gul O, Sezerman U. Effect of codon optimization on the expression of Trichoderma reesei endoglucanase 1 in Pichia pastoris. Biotechnol Prog. 2011 Sep-Oct;27(5):1257-63. doi: 10.1002/btpr.663. Epub 2011 Jul 19. PubMed PMID: 21774095.
- Stadlmayr G, Mecklenbräuker A, Rothmüller M, Maurer M, Sauer M, Mattanovich D, Gasser B. Identification and characterisation of novel Pichia pastoris promoters for heterologous protein production. J Biotechnol. 2010 Dec;150(4):519-29. Epub 2010 Oct 8. PubMed PMID: 20933554.
- De Pourcq K, De Schutter K, Callewaert N. Engineering of glycosylation in yeast and other fungi: current state and perspectives. Appl Microbiol Biotechnol. 2010 Aug;87(5):1617-31. Epub 2010 Jun 29. Review. PMID: 20585772.
- Guerfal M, Ryckaert S, Jacobs PP, Ameloot P, Van Craenenbroeck K, Derycke R, Callewaert N. The HAC1 gene from Pichia pastoris: characterization and effect of proteins. Microb Cell Fact. 2010 Jun 30;9:49. PubMed PMID: 20591165; PubMed Central PMCID: PMC2905327.
- Al-Samarrai TH, Kirk CA, Jones WT, Harvey D, Templeton MD. A simple and rapid method for selecting high producers of recombinant proteins in individual clones of P. pastoris. Comb Chem High Throughput Screen. 2010 Jun;13(5):377-82. PubMed PMID: 20236060.
- Vanderschaeghe D, Festjens N, Delanghe J, Callewaert N. Glycome profiling using modern glycomics technology: technical aspects and applications. Biol Chem. 2010 Feb-Mar;391(2-3):149-61. Review. PMID: 20128687.
- Jacobs PP, Geysens S, Vervecken W, Contreras R, Callewaert N. Engineering complex-type N-glycosylation in Pichia pastoris using GlycoSwitch technology. Nat Protoc. 2009;4(1):58-70. PubMed PMID: 19131957.
- Jacobs PP, Ryckaert S, Geysens S, De Vusser K, Callewaert N, Contreras R.Pichia surface display: display of proteins on the surface of glycoengineered Pichia pastoris strains. Biotechnol Lett. 2008 Dec;30(12):2173-81. Epub 2008 Aug 5. PubMed PMID: 18679585.
- Blanchard V, Gadkari RA, George AV, Roy S, Gerwig GJ, Leeflang BR, Dighe RR, Boelens R, Kamerling JP. High-level expression of biologically active glycoprotein hormones in Pichia pastoris strains–selection of strain GS115, and not X-33, for the production of biologically active N-glycosylated 15N-labeled phCG. Glycoconj J. 2008 Apr;25(3):245-57. Epub 2008 Feb 15. PubMed PMID: 18274893; PubMed Central PMCID: PMC2668595.
- Lin-Cereghino J, Lin-Cereghino GP. Vectors and strains for expression. Methods Mol Biol. 2007;389:11-26. PubMed PMID: 17951632.
- Cregg JM. Introduction: distinctions between Pichia pastoris and other expression systems. Methods Mol Biol. 2007;389:1-10. Review. PubMed PMID: 17951631.
- Vervecken W, Callewaert N, Kaigorodov V, Geysens S, Contreras R. Modification of the N-glycosylation pathway to produce homogeneous, human-like glycans using GlycoSwitch plasmids. Methods Mol Biol. 2007;389:119-38. PMID: 17951639.
- Huo X, Liu Y, Wang X, Ouyang P, Niu Z, Shi Y, Qiu B. Co-expression of human protein disulfide isomerase (hPDI) enhances secretion of bovine follicle-stimulating hormone (bFSH) in Pichia pastoris. Protein Expr Purif. 2007 Aug;54(2):234-9. Epub 2007 Mar 31. PubMed PMID: 17498970.
- Hamilton SR, Davidson RC, Sethuraman N, Nett JH, Jiang Y, Rios S, Bobrowicz P, Stadheim TA, Li H, Choi BK, Hopkins D, Wischnewski H, Roser J, Mitchell T, Strawbridge RR, Hoopes J, Wildt S, Gerngross TU. Humanization of yeast to produce complex terminally sialylated glycoproteins. Science. 2006 Sep 8;313(5792):1441-3. PubMed PMID: 16960007.
- Vervecken W, Kaigorodov V, Callewaert N, Geysens S, De Vusser K, Contreras R. In vivo synthesis of mammalian-like, hybrid-type N-glycans in Pichia pastoris. Appl Environ Microbiol. 2004 May;70(5):2639-46. PubMed PMID: 15128513; PubMed Central PMCID: PMC404441.